uMelt Quartz

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Thermodynamics
Free [Mg++]mM
[Mono+] mM
DMSO%
Salt Correction
Resolution

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Parameter Definitions

[Mono+]: Concentration of monovalent cations.

Free [Mg++]: Final concentration after dNTPs are subtracted from [Mg++]

Thermodynamic Library: Published nearest neighbor parameters to use in calculation.

Salt Correction: Algorithm for [Mono+] and [Mg++] corrections to use in calculation. Currently hard set to use:

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Contact Information

Questions or feedback?: zach.dwight@path.utah.edu

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About uMelt

Version: Release: 3.6.2 "Quartz" / Nov 5 2020

User Guide & Instructions: Link to PDF

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Cite uMelt

Zachary Dwight, Robert Palais, Carl T. Wittwer, uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application, Bioinformatics, Volume 27, Issue 7, 1 April 2011, Pages 1019–1020, LINK TO MANUSCRIPT