|About | Publication | Definitions | Contact Us|
[Mono+]: Concentration of monovalent cations.
Free [Mg++]: Final concentration after dNTPs are subtracted from [Mg++]
Thermodynamic Library: Published nearest neighbor parameters to use in calculation.
Salt Correction: Algorithm for [Mono+] and [Mg++] corrections to use in calculation. Currently hard set to use:
Questions or feedback?: firstname.lastname@example.org
Version: Release: 3.6.2 "Quartz" / Nov 5 2020
User Guide & Instructions: Link to PDF
Zachary Dwight, Robert Palais, Carl T. Wittwer, uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application, Bioinformatics, Volume 27, Issue 7, 1 April 2011, Pages 1019–1020, LINK TO MANUSCRIPT