Parameter Definitions

[Mono+]: Concentration of monovalent cations.

Free [Mg++]: Final concentration after dNTPs are subtracted from [Mg++]

Thermodynamic Library: Published nearest neighbor parameters to use in calculation.

Salt Correction: Algorithm for [Mono+] and [Mg++] corrections to use in calculation. Currently hard set to use:

• von Ahsen, N., Wittwer, C. T., & Schutz, E. (2001).
  Oligonucleotide melting temperatures under PCR conditions [cont...], Clin Chem, 47(11), 1956+.
• SantaLucia J Jr, Hicks D. (2004).
  The thermodynamics of DNA structural motifs. Annu Rev Biophys Biomol Struct. 33:415-440.

Contact Information

Questions or feedback?: zach.dwight@path.utah.edu

About uMelt Batch

Version: Release: 3.6.2 "Quartz" / Nov 5 2020

User Guide & Instructions: Link to PDF

Cite uMelt

Zachary Dwight, Robert Palais, Carl T. Wittwer, uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application, Bioinformatics, Volume 27, Issue 7, 1 April 2011, Pages 1019–1020, LINK TO MANUSCRIPT